Description
Kanamycin is an aminoglycoside antibiotic that binds to 30S ribosomes to cause misreading of mRNA codes, thereby inhibiting protein synthesis. It is effective against both Gram-positive bacteria and Gram-negative bacteria. Kanamycin can be used in cell culture to inhibit bacterial contamination. In molecular biology research, it is often used to selectively screen bacterial clones that have successfully transformed the kanamycin resistance gene. It is also commonly used in agrobacterium-mediated transformation experiments to selectively screen plant tissues carrying npt II (APH3) genes